Use of bovine sex sorted sperm on timed artificial insemination, in vivo and in vitro embryo production programs

نویسندگان

  • P. S. Baruselli
  • J. G. Soares
  • J.N.S. Sales
  • G. A. Crepaldi
  • A. H. Souza
  • K.A.L. Neves
  • C. M. Martins
  • E. P. Campos Filho
  • M. F. Sá Filho
  • M. Bertolini
  • J. L. Rodrigues
چکیده

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Sex-sorted sperm for artificial insemination and embryo transfer programs in cattle

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Pregnancy rates of lactating cows after transfer of in vitro produced embryos using X-sorted sperm.

The main objective was to determine the efficacy of using X-sorted sperm to produce embryos in vitro for transfer into lactating dairy cows. Cows were bred by timed artificial insemination (TAI) using nonsorted semen or X-sorted sperm, or they received a fresh embryo produced in vitro by fertilization with X-sorted or nonsorted sperm using timed embryo transfer (TET). Pregnancy rates at approxi...

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An Overview of Sex Selection at Conception in Mammals

Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...

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Effects on glycocalyx structures of frozen-thawed bovine sperm induced by flow cytometry and artificial capacitation

Sperm sorting by flow cytometry is a useful technology in the bovine industry, but the conception rates after artificial insemination using sex-sorted sperm are lower than when using the un-sorted sperm. In this study, we have investigated the causes for these low conception rates. We have focused on changes caused by flow cytometry to the glycocalyx, which forms the outermost surface of the sp...

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Integration of sperm sexing technology into the ART toolbox.

Sex-sorting of mammalian spermatozoa has applications for genetic improvement of farm animals, in humans for the control of sex-linked disease, and in wildlife as a captive management strategy and for the re-population of endangered species. Considerable research has been undertaken worldwide on the Beltsville sperm sexing technology, the only effective method for pre-selection of sex of offspr...

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تاریخ انتشار 2013